Why paint fabrics … the person? A histochemistry and cytochemistry of
the Histochemistry , the section of histology (from Greek ί σ τ ί ο μ - fabric and Greek Λ ό γ ο ς - knowledge, the word, science) - the section of biology on a structure of fabrics of live organisms), studying localization of various chemicals and products of their metabolism in fabrics. The histochemistry provides communication between biochemistry and morphology.
Some methods of coloring allow to reveal these or those chemicals in cages. Perhaps differential coloring of fats, glycogen, nucleinic acids, nukleoprotein, certain enzymes and other chemical components of a cage. The histochemistry contribution to studying of a chemical composition of fabrics constantly increases. Dyes, fluorokhroma and enzymes which can be attached to specific immunoglobulins (antibodies) are picked up and, observing binding of this complex in a cage, to identify cellular structures. This area of researches makes a subject of an immunohistochemistry . Use of immunological markers in light and electronic microscopy promotes expansion of knowledge of cytobiology, and also increase of accuracy of medical diagnoses.
Optical coloring . Traditional histologic methods of coloring are accompanied by fixing which kills fabrics. Methods of optical coloring are based that the cages and fabrics differing on thickness and a chemical composition possess also different optical properties. As a result, using the polarized light, dispersion, an interference or phase contrast, it is possible to receive images on which details of a structure are well visible thanks to distinctions in brightness and (or) coloring whereas in a usual light microscope such details of a malorazlichima. These methods allow to study both the live, and fixed fabrics. By means of histochemical techniques it was succeeded to show for the first time connection of changes of quantity of RNA with synthesis of protein and constancy of maintenance of DNA in a set of chromosomes.
The lab develops at Institute of theoretical and experimental biophysics of the Russian Academy of Sciences. functional histochemistry (Pushchino); Scientific Research Institute of Emergency Medicine of N. V. Sklifosovsky, lab. patomorfologiya with a histochemistry (Moscow). It is possible to esteem: Luppa X. Histochemistry bases. M.: World, 1980.
The term the cytochemistry is applied in literature in two values - or as a cage ultrahistochemistry or as the sum of the microscopic methods allowing to carry out the chemical and enzymatic analysis of cages or groups of cages at preservation of their morphology.
The cytochemistry studies by chemical methods a structure and functions of cages, intracellular structures and products of their activity. The term “cage“ is offered by the English physicist R. Guk in 1665 which was her pioneer.
In a human body about 10 14 cages of various types. The substances dissolved in cellular juice of plants (sugar, polysaccharides, alkaloids, tannins, pigments, some salts, etc.) cause owing to osmosis receipt in cells of nutrients and water and create the mechanical tension of cages and fabrics - turgor .
By means of osmosis water comes to cages of all vegetable and animal organisms. In fabrics of plants osmotic pressure is 0,5 - 2 MPas (at plants in deserts more than 10 MPas) - is the main reason of rising of water from roots to tops . At 37 °C osmotic pressure is equal in blood of the person to 0,78 MPas (7,7 atm.). The feeling of thirst is caused by need of an organism for water for restoration of normal osmotic pressure in cages after it was raised, for example, by the use of salty food.
Deal with issues of cytochemistry at Institute of cytology of the Russian Academy of Sciences (St. Petersburg), Institute of cytology and genetics of the Siberian Branch of the Russian Academy of Science (Novosibirsk). There is a Tsitologiya magazine. It is possible to esteem: Brodsky V. Ya., Polyakov N. I. Introduction to quantitative cytochemistry. M.: 1969.